hek 293ft human embryonic kidney cells Search Results


human embryonic kidney cell line 293ft  (ScienCell)
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ScienCell human embryonic kidney cell line 293ft
( A ) Immunofluorescence analysis of AQP7 expression in mouse oocytes in the presence of 0.25 M, 0.5 M, 0.75 M and 1 M sucrose, respectively. ( B ) Summary data of the immunofluorescence analysis (n ≥ 5). ( C ) Immunofluorescence analysis of AQP7 and F-actin colocalization in mouse oocytes (red: AQP7; green: F-actin). Scale bar ( A – C ), 20 μm, ( D ) Co-immunoprecipitation of AQP7 and F-actin. IP, immunoprecipitation. ( E ) Green fluorescent protein (GFP) intensity in <t>293FT</t> cells transfected with the GFP-hAQP7 fusion protein expression plasmid or with the GFP vector alone in the presence of 8% EG, 9.5% DMSO, and 0.5 M sucrose, respectively. Scale bar, 10 μm. ( F ) Summary data of the immunofluorescence analysis (calculated for 20 cells for each condition obtained from three independent experiments). Data are presented as the mean ± SE, ** P < 0.01 compared to the corresponding control.
Human Embryonic Kidney Cell Line 293ft, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Immunofluorescence analysis of AQP7 expression in mouse oocytes in the presence of 0.25 M, 0.5 M, 0.75 M and 1 M sucrose, respectively. ( B ) Summary data of the immunofluorescence analysis (n ≥ 5). ( C ) Immunofluorescence analysis of AQP7 and F-actin colocalization in mouse oocytes (red: AQP7; green: F-actin). Scale bar ( A – C ), 20 μm, ( D ) Co-immunoprecipitation of AQP7 and F-actin. IP, immunoprecipitation. ( E ) Green fluorescent protein (GFP) intensity in 293FT cells transfected with the GFP-hAQP7 fusion protein expression plasmid or with the GFP vector alone in the presence of 8% EG, 9.5% DMSO, and 0.5 M sucrose, respectively. Scale bar, 10 μm. ( F ) Summary data of the immunofluorescence analysis (calculated for 20 cells for each condition obtained from three independent experiments). Data are presented as the mean ± SE, ** P < 0.01 compared to the corresponding control.

Journal: Scientific Reports

Article Title: Aquaporin7 plays a crucial role in tolerance to hyperosmotic stress and in the survival of oocytes during cryopreservation

doi: 10.1038/srep17741

Figure Lengend Snippet: ( A ) Immunofluorescence analysis of AQP7 expression in mouse oocytes in the presence of 0.25 M, 0.5 M, 0.75 M and 1 M sucrose, respectively. ( B ) Summary data of the immunofluorescence analysis (n ≥ 5). ( C ) Immunofluorescence analysis of AQP7 and F-actin colocalization in mouse oocytes (red: AQP7; green: F-actin). Scale bar ( A – C ), 20 μm, ( D ) Co-immunoprecipitation of AQP7 and F-actin. IP, immunoprecipitation. ( E ) Green fluorescent protein (GFP) intensity in 293FT cells transfected with the GFP-hAQP7 fusion protein expression plasmid or with the GFP vector alone in the presence of 8% EG, 9.5% DMSO, and 0.5 M sucrose, respectively. Scale bar, 10 μm. ( F ) Summary data of the immunofluorescence analysis (calculated for 20 cells for each condition obtained from three independent experiments). Data are presented as the mean ± SE, ** P < 0.01 compared to the corresponding control.

Article Snippet: The human embryonic kidney cell line (293FT) was purchased from ScienCell (Carlsbad, USA), and cultured in DMEM (Gibco, Grand Island, USA) containing 10% fetal bovine serum (Gibco).

Techniques: Immunofluorescence, Expressing, Immunoprecipitation, Transfection, Plasmid Preparation, Control

( A ) Oocytes were pretreated with LY294002, staurosporine, U0126 and SP600125, respectively, and the immunofluorescence intensities of AQP7 were analysed in mouse oocytes in the presence of 8% EG. Scale bar, 20 μm. ( B ) Summary data of the immunofluorescence analysis (n ≥ 7). ( C ) 293FT cells were transfected with the GFP-hAQP7 fusion protein expression plasmid and treated as in ( A ). The immunofluorescence intensities of GFP-hAQP7 were analysed in the presence of 8% EG. Scale bar, 10 μm. ( D ) Summary data of the immunofluorescence analysis (calculated for 20 cells for each condition obtained from three independent experiments). ( E ) Western blotting analysis of GFP-hAQP7 in 293FT cells treated as in ( C ). ( F ) Summary data of the Western blotting analysis (n = 3). Data are presented as the mean ± SE. ** P < 0.01 compared to the corresponding control.

Journal: Scientific Reports

Article Title: Aquaporin7 plays a crucial role in tolerance to hyperosmotic stress and in the survival of oocytes during cryopreservation

doi: 10.1038/srep17741

Figure Lengend Snippet: ( A ) Oocytes were pretreated with LY294002, staurosporine, U0126 and SP600125, respectively, and the immunofluorescence intensities of AQP7 were analysed in mouse oocytes in the presence of 8% EG. Scale bar, 20 μm. ( B ) Summary data of the immunofluorescence analysis (n ≥ 7). ( C ) 293FT cells were transfected with the GFP-hAQP7 fusion protein expression plasmid and treated as in ( A ). The immunofluorescence intensities of GFP-hAQP7 were analysed in the presence of 8% EG. Scale bar, 10 μm. ( D ) Summary data of the immunofluorescence analysis (calculated for 20 cells for each condition obtained from three independent experiments). ( E ) Western blotting analysis of GFP-hAQP7 in 293FT cells treated as in ( C ). ( F ) Summary data of the Western blotting analysis (n = 3). Data are presented as the mean ± SE. ** P < 0.01 compared to the corresponding control.

Article Snippet: The human embryonic kidney cell line (293FT) was purchased from ScienCell (Carlsbad, USA), and cultured in DMEM (Gibco, Grand Island, USA) containing 10% fetal bovine serum (Gibco).

Techniques: Immunofluorescence, Transfection, Expressing, Plasmid Preparation, Western Blot, Control